Cristina N, Chatellard-Causse C, Manier M, Feuerstein C
Laboratoire de Physiologie, Inserm U318, C.H.U. de Grenoble, France.
Brain Res Mol Brain Res. 1995 Sep;32(2):354-7. doi: 10.1016/0169-328x(95)00103-y.
The detection of the glial cell-line derived neurotrophic factor (GDNF) mRNA by RT-PCR in dissociated cell culture of rat embryonic or post-natal brain allowed the amplification of a doublet. The major band corresponded to the expected size and the minor one to a shorter product. We cloned and sequenced the latter product, and thus identified a mRNA potentially encoding for an isoform of the initially described precursor protein involved in GDNF synthesis.
通过逆转录聚合酶链反应（RT-PCR）在大鼠胚胎或出生后脑的解离细胞培养物中检测胶质细胞系源性神经营养因子（GDNF）mRNA，得到了一个双峰扩增产物。主要条带对应预期大小，次要条带对应较短产物。我们对后者产物进行了克隆和测序，从而鉴定出一种可能编码参与GDNF合成的最初描述的前体蛋白异构体的mRNA。
