Suter-Crazzolara C, Unsicker K
Neuroreport. 1994 Dec 20;5(18):2486-8. doi: 10.1097/00001756-199412000-00020.
A recently cloned neurotrophic factor, Glial Cell Line-Derived Neurotrophic Factor (GDNF), has been implicated in the survival and morphological and functional differentiation of midbrain dopaminergic neurones in vitro. GDNF has therefore been proposed as a factor which may have utility in the treatment of Parkinson's disease. In the present study, we have used RT-PCR to analyse the distribution of GDNF mRNA throughout the newborn rat (P0). We show that GDNF transcripts are present in kidney, lung, bone, heart, liver, spleen, sciatic nerve and blood. Two separate GDNF transcripts are present in different ratios in each tissue investigated. Sequence analysis of both these mRNA species revealed that the shorter transcript (sGDNF) contains a deletion of 78 bp in comparison to the published sequence for GDNF. We speculate that this shorter mRNA arose due to alternative splicing.
最近克隆出的一种神经营养因子，即胶质细胞系源性神经营养因子（GDNF），已被证明在体外对中脑多巴胺能神经元的存活以及形态和功能分化具有重要作用。因此，GDNF被认为是一种可能对帕金森病治疗有用的因子。在本研究中，我们利用逆转录聚合酶链反应（RT-PCR）分析了新生大鼠（出生0天，P0）全身GDNF信使核糖核酸（mRNA）的分布情况。我们发现，GDNF转录本存在于肾脏、肺、骨骼、心脏、肝脏、脾脏、坐骨神经和血液中。在所研究的每个组织中，两种不同的GDNF转录本以不同比例存在。对这两种mRNA的序列分析表明，与已公布的GDNF序列相比，较短的转录本（sGDNF）缺失了78个碱基对。我们推测，这种较短的mRNA是由于选择性剪接产生的。
