酵母起始识别复合体的一种果蝇同源物。

A Drosophila homolog of the yeast origin recognition complex.

作者信息

Gossen M, Pak D T, Hansen S K, Acharya J K, Botchan M R

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.

出版信息

Science. 1995 Dec 8;270(5242):1674-7. doi: 10.1126/science.270.5242.1674.

DOI:10.1126/science.270.5242.1674

PMID:7502079

Abstract

Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.

摘要

已经鉴定出黑腹果蝇中编码与酿酒酵母起源识别复合物（ORC）的Orc2p和Orc5p同源蛋白质的基因。果蝇Orc2p同源物DmORC2的丰度受发育调控，在胚胎发生的最早阶段最高，这与最高的DNA复制速率同时出现。胚胎核提取物的分级分离显示，DmORC2与另外五种多肽形成紧密相关的复合物，这与酵母ORC非常相似。这些研究将能够直接测试后生动物中基于引发剂的复制模型。