Sun X W, Ferenczy A, Johnson D, Koulos J P, Lungu O, Richart R M, Wright T C
Department of Pathology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Am J Obstet Gynecol. 1995 Nov;173(5):1432-7. doi: 10.1016/0002-9378(95)90629-0.
Our purpose was to evaluate the sensitivity and accuracy of a new, nonradioactive human papillomavirus deoxyribonucleic acid detection method.
Cervical samples from 520 women were assayed for human papillomavirus deoxyribonucleic acid with both the Hybrid Capture test and polymerase chain reaction.
Human papillomavirus deoxyribonucleic acid was detected with Hybrid Capture in 106 (42%) of 254 samples from women with no evidence of cervical intraepithelial neoplasia and 211 (79%) of 266 with cervical intraepithelial lesions or cervical cancer. There was a good correlation between Hybrid Capture and polymerase chain reaction. Hybrid Capture correctly identified 92% of samples found to contain a human papillomavirus type with a high or intermediate oncogenic risk with polymerase chain reaction. Although Hybrid Capture can quantify the amount of human papillomavirus deoxyribonucleic acid present in a sample, no correlation was observed between the relative amount of human papillomavirus deoxyribonucleic acid detected with Hybrid Capture and the grade of cervical lesion.
The Hybrid Capture test is a sensitive and accurate method for identifying human papillomavirus types of high and intermediate oncogenic risk in clinical specimens.
我们的目的是评估一种新的非放射性人乳头瘤病毒脱氧核糖核酸检测方法的敏感性和准确性。
对520名女性的宫颈样本进行检测，采用杂交捕获试验和聚合酶链反应检测人乳头瘤病毒脱氧核糖核酸。
在254例无宫颈上皮内瘤变证据的女性样本中，杂交捕获法检测到人乳头瘤病毒脱氧核糖核酸的有106例（42%）；在266例有宫颈上皮内病变或宫颈癌的女性样本中，检测到的有211例（79%）。杂交捕获试验和聚合酶链反应之间存在良好的相关性。杂交捕获试验正确识别了92%经聚合酶链反应检测发现含有高致癌风险或中致癌风险人乳头瘤病毒类型的样本。虽然杂交捕获试验可以对样本中存在的人乳头瘤病毒脱氧核糖核酸量进行定量，但未观察到杂交捕获试验检测到的人乳头瘤病毒脱氧核糖核酸相对量与宫颈病变分级之间存在相关性。
杂交捕获试验是一种在临床标本中识别高致癌风险和中致癌风险人乳头瘤病毒类型的敏感且准确的方法。
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