Hasegawa K, Tanaka M, Kobayashi H, Kikuno M
Nihon Yakurigaku Zasshi. 1978 Nov;74(8):941-50. doi: 10.1254/fpj.74.941.
In an analysis of the gastrocnemius muscle, the microsomal fraction showed the highest cholinesterase (ChE) activity. The ChE activity of all fractions decreased to a greater extent after strong nerve crushing than after weak crushing. This change in the activity in the microsomal fraction was the most marked change observed. Although in the analysis of the soleus muscle the ChE activity was measured only in the homogenate and in the microsomal fraction, the results were the same as those obtained with the gastrocnemius. A preparation of vitamins B1, B2, and B12 (B complex) had little effect on the ChE activity in the gastrocnemius muscle. In the soleus muscle on the lesion side, the B complex increased the ChE activity to some extent after nerve crushing, but such was not significant. However, the B complex signifiicantly increased this activity in the soleus on the intact side. In the soleus muscle, strong nerve crushing induced more marked muscle atrophy than weak crushing. On the other hand, no significant difference was found in the gastrocnemius. Effects of the B complex on muscle atrophy were found in the soleus, but not in the gastrocnemius. These results suggest that the ChE activity in the microsomal fraction containing sarcoplasmic reticulum reflects the nervous disorders clearly, and that the B complex increases the ChE activity and muscle weight in the soleus, but not in the gastrocnemius muscle.
在对腓肠肌的分析中，微粒体部分显示出最高的胆碱酯酶（ChE）活性。与轻度神经挤压后相比，重度神经挤压后所有部分的ChE活性下降幅度更大。微粒体部分活性的这种变化是观察到的最显著变化。尽管在比目鱼肌的分析中，仅在匀浆和微粒体部分测量了ChE活性，但结果与腓肠肌的结果相同。维生素B1、B2和B12（复合维生素B）制剂对腓肠肌中的ChE活性几乎没有影响。在损伤侧的比目鱼肌中，复合维生素B在神经挤压后使ChE活性有所增加，但不显著。然而，复合维生素B显著增加了完整侧比目鱼肌的这种活性。在比目鱼肌中，重度神经挤压比轻度挤压引起更明显的肌肉萎缩。另一方面，在腓肠肌中未发现显著差异。复合维生素B对肌肉萎缩的影响在比目鱼肌中可见，但在腓肠肌中未见。这些结果表明，含有肌浆网的微粒体部分中的ChE活性清楚地反映了神经紊乱，并且复合维生素B增加了比目鱼肌中的ChE活性和肌肉重量，但在腓肠肌中则不然。
