Zanlungo S, Bégu D, Quiñones V, Araya A, Jordana X
Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Casilla, Santiago.
Curr Genet. 1993 Oct;24(4):344-8. doi: 10.1007/BF00336787.
Editing of the complete coding region of cob transcripts from two genera of plants has been studied by cDNA sequence analysis. Eighteen and nine C residues are edited into U in the mitochondrial transcripts from wheat and potato respectively. Both systems share eight common editing sites; ten codons edited in wheat are "pre-edited" at the genomic level in potato, and one codon edited in potato is "pre-edited" in wheat. Most amino-acid modifications lead to hydrophobic residues and increase the homology between the COB polypeptides and the corresponding protein of other species. In two out of the nine potato cDNA clones, an additional C-to-T modification, which also leads to a change in the encoded amino acid, was identified. Heterogeneity observed at the carboxy-terminus of the COB open reading frame in Triticum aestivum and Triticum timopheevi is not corrected by editing.
通过cDNA序列分析，对两种植物属的cob转录本完整编码区的编辑情况进行了研究。在小麦和马铃薯的线粒体转录本中，分别有18个和9个C残基被编辑为U。这两个系统共有8个共同的编辑位点；在小麦中编辑的10个密码子在马铃薯的基因组水平上是“预编辑”的，而在马铃薯中编辑的1个密码子在小麦中是“预编辑”的。大多数氨基酸修饰导致疏水残基，并增加了COB多肽与其他物种相应蛋白质之间的同源性。在9个马铃薯cDNA克隆中的2个中，鉴定出了额外的C到T修饰，这也导致了编码氨基酸的变化。普通小麦和提莫菲维小麦COB开放阅读框羧基末端观察到的异质性未通过编辑得到校正。
