Brandt P, Unseld M, Eckert-Ossenkopp U, Brennicke A
Institut für Genbiologische Forschung, Berlin, Germany.
Curr Genet. 1993 Oct;24(4):330-6. doi: 10.1007/BF00336785.
Sequence analysis of the region upstream of the apocytochrome b (cob) gene in the Arabidopsis mitochondrial genome identifies an open reading frame with homology to ribosomal protein L5, (rpl5), and a pseudogene with similarity to ribosomal protein S14 (rps14) genes. Both cob and rpl5 genes have intact reading frames, but the rps14 homology is disrupted by a stop codon and a deleted nucleotide. The rpl5 gene, the rps14 pseudogene, and the cob gene are separated by one nucleotide and a 1604-nucleotide-long spacer respectively. A plastid-like tRNA(Ser) is encoded downstream from the cob gene. The entire region is transcribed into a 5-kb transcript, containing the rps14 pseudogene and the cob gene. Cob and rpl5 mRNAs are edited in several positions with different frequencies. The rps14 pseudogene is transcribed and edited in one position in common with other plants. Since no intact rps14 gene is found in the mitochondrial genome of Arabidopsis, the functional gene is presumably encoded in the nucleus.
对拟南芥线粒体基因组中脱辅基细胞色素b（cob）基因上游区域进行序列分析，鉴定出一个与核糖体蛋白L5（rpl5）具有同源性的开放阅读框，以及一个与核糖体蛋白S14（rps14）基因相似的假基因。cob和rpl5基因都有完整的阅读框，但rps14的同源性被一个终止密码子和一个缺失的核苷酸破坏。rpl5基因、rps14假基因和cob基因分别被一个核苷酸和一个1604个核苷酸长的间隔区隔开。一个类质体的tRNA(Ser)编码在cob基因的下游。整个区域转录成一个5 kb的转录本，包含rps14假基因和cob基因。Cob和rpl5的mRNA在几个位置以不同频率进行编辑。rps14假基因在一个位置转录并编辑，这与其他植物相同。由于在拟南芥的线粒体基因组中未发现完整的rps14基因，推测功能基因编码在细胞核中。
