针对单纯疱疹病毒1型中和单克隆抗体的单克隆抗独特型抗体：制备与特性鉴定

Monoclonal anti-idiotype antibody to HSV-1 neutralizing monoclonal antibody: production and characterization.

作者信息

Dar V S, Seth P

机构信息

Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.

出版信息

Dis Markers. 1993 Sep;11(2-3):113-23. doi: 10.1155/1993/564957.

DOI:10.1155/1993/564957

PMID:7505213

Abstract

This study is an attempt to produce and characterize murine monoclonal antibodies directed against the paratope of HSV-1 neutralizing monoclonal antibody. Monoclonal antibody 138 C5G10 which was neutralising and directed to 120 K antigen gB of HSV-1 was used as the idiotype. We were able to produce three Ab-2 monoclonal antibodies as characterized using immunofluorescence, ELISA and RIA. The findings of the present study suggest that two antiidiotypes 3AiB3E10 and 3AiB5D10 share the same unique fine specificity while 3AiB3C9 has a different specificity on 138 C5G10 paratope. The utility of such 'surrogate' antigens in serological assays and modulation of immune response is discussed.

摘要

本研究旨在制备并鉴定针对单纯疱疹病毒1型（HSV-1）中和性单克隆抗体互补决定区的小鼠单克隆抗体。将具有中和活性且针对HSV-1 120K抗原gB的单克隆抗体138 C5G10用作独特型。利用免疫荧光、酶联免疫吸附测定（ELISA）和放射免疫分析（RIA）进行鉴定，我们成功制备了三种抗独特型单克隆抗体。本研究结果表明，两种抗独特型抗体3AiB3E10和3AiB5D10具有相同的独特精细特异性，而3AiB3C9对138 C5G10互补决定区具有不同的特异性。本文还讨论了此类“替代”抗原在血清学检测及免疫反应调节中的应用。