Morrow W J, Gaston I, Anderson T, Haigwood N, McGrath M S, Rosen J, Steimer K S
Department of Laboratory Medicine, University of California, San Francisco.
Viral Immunol. 1990 Summer;3(2):99-109. doi: 10.1089/vim.1990.3.99.
One potential strategy for the control of human immunodeficiency virus (HIV) infection is immune network manipulation using anti-idiotypic antibodies: this study was undertaken to demonstrate experimentally the potential of such an approach which, in a more highly evolved form, could be used for the treatment of the acquired immune deficiency virus (AIDS) and related disorders. Anti-idiotypic antibodies were generated in rabbits against a murine monoclonal antibody identifying an epitope on the p24 gag core protein of HIV. After extensive absorption on affinity columns to remove isotype- and allotype-specific antibodies, the purified anti-idiotypic antibody preparation was shown to have specific complementarity with the immunizing mouse monoclonal antibody. This anti-idiotypic antibody was also shown to recognize a common idiotype associated with HIV-specific antibodies from both humans and chimpanzees infected with the AIDS virus. In addition a group of rats immunized with the anti-Id responded with significant antibody titers to recombinant derived p24 gag. These data indicate that at least a subpopulation of these polyclonal anti-Id antibodies structurally mimics an HIV gag region epitope and suggest that immunoregulation by anti-idiotypic antibodies may have therapeutic utility for the AIDS epidemic.
控制人类免疫缺陷病毒（HIV）感染的一种潜在策略是使用抗独特型抗体来操纵免疫网络：本研究旨在通过实验证明这种方法的潜力，在更高级的形式下，该方法可用于治疗获得性免疫缺陷病毒（AIDS）及相关疾病。针对一种识别HIV p24 gag核心蛋白上一个表位的鼠单克隆抗体，在兔体内产生了抗独特型抗体。在亲和柱上进行广泛吸附以去除同种型和异型特异性抗体后，纯化的抗独特型抗体制剂显示与免疫用小鼠单克隆抗体具有特异性互补性。该抗独特型抗体还被证明能识别与感染艾滋病病毒的人类和黑猩猩体内的HIV特异性抗体相关的一种共同独特型。此外，一组用抗独特型抗体免疫的大鼠对重组来源的p24 gag产生了显著的抗体滴度。这些数据表明，这些多克隆抗独特型抗体中至少有一个亚群在结构上模拟了HIV gag区域的一个表位，并表明抗独特型抗体的免疫调节可能对艾滋病流行具有治疗作用。
