Guber A, Cohen R, Ronah R, Zan Bar I, Geiger B, Bruderman I
Chest Department, Sapir Medical Center, Meir General Hospital, Kfar Saba, Israel.
Chest. 1994 Jan;105(1):138-43. doi: 10.1378/chest.105.1.138.
In the current study a comparative analysis of keratin typing and DNA content was carried out in human lung tumors from transthoracic fine needle aspiration biopsies (TFNAB) (18 patients) or from surgically resected tumor tissues (14 patients). According to the cytologic and histologic features, 2 of the 32 tumors were diagnosed as benign tumors, 11 as squamous cell carcinomas, 12 as adenocarcinomas, and 7 as undifferentiated large cell carcinomas. Two cases in the adenocarcinoma and one in the undifferentiated large cell carcinoma groups were pulmonary metastasis or second primary tumors. Malignant cells of tumors which reacted positively with KK8.60 anticytokeratin polypeptides No. 10 and 11 (and hence contain keratinizing cells) displayed diploid DNA content in a flow cytometric assay regardless of their cytologic or histologic appearance. In contrast, all tumors which lacked such positive cells (most of which were defined as adenocarcinomas and undifferentiated tumors) were hyperdiploid. The close correlation between high DNA content and both malignancy and the absence of advanced squamous differentiation (keratinization) suggests that such combined analysis may provide new tools for the cytologic diagnosis and prognosis of lung cancers.
在本研究中，对经胸细针穿刺活检（TFNAB）（18例患者）或手术切除肿瘤组织（14例患者）获得的人肺肿瘤进行了角蛋白分型和DNA含量的比较分析。根据细胞学和组织学特征，32例肿瘤中有2例被诊断为良性肿瘤，11例为鳞状细胞癌，12例为腺癌，7例为未分化大细胞癌。腺癌组中有2例和未分化大细胞癌组中有1例为肺转移瘤或第二原发性肿瘤。与KK8.60抗细胞角蛋白多肽10和11呈阳性反应（因此含有角化细胞）的肿瘤恶性细胞，在流式细胞术检测中显示二倍体DNA含量，无论其细胞学或组织学表现如何。相反，所有缺乏此类阳性细胞的肿瘤（其中大多数被定义为腺癌和未分化肿瘤）均为超二倍体。高DNA含量与恶性程度以及缺乏高级别鳞状分化（角化）之间的密切相关性表明，这种联合分析可能为肺癌的细胞学诊断和预后提供新的工具。
