多聚体载体：一种使用λ噬菌体包装提取物包装丝状噬菌体和噬菌粒载体的系统。
Polycos vectors: a system for packaging filamentous phage and phagemid vectors using lambda phage packaging extracts.
作者信息
Alting-Mees M A, Short J M
机构信息
Stratagene, La Jolla, CA 92037.
出版信息
Gene. 1993 Dec 27;137(1):93-100. doi: 10.1016/0378-1119(93)90256-3.
A new class of hybrid vectors, 'polycos' vectors, incorporate a phage lambda cos site and filamentous phage origin to allow high-efficiency cloning via in vitro lambda packaging extracts. The head-filling mechanism of cos site recognition by lambda packaging proteins permits concatemers of several of these small cos-containing vectors to be packaged per phage particle. Excision of vector monomers after infection is accomplished via a lambda ZAP-like M13 excision process. This system has the advantage of adapting high-efficiency lambda packaging extracts to M13 and phagemid cloning vectors.
一类新型的杂种载体——“多cos”载体，整合了噬菌体λ cos位点和丝状噬菌体复制起点，以允许通过体外λ包装提取物进行高效克隆。λ包装蛋白对cos位点识别的头部填充机制使每个噬菌体颗粒能够包装几个这类含小cos的载体的串联体。感染后载体单体的切除通过类似λ ZAP的M13切除过程完成。该系统具有使高效λ包装提取物适用于M13和噬菌粒克隆载体的优点。
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