λZLG6：一种用于cDNA文库在丝状噬菌体上进行高效克隆和表面表达的λ噬菌体载体。

LambdaZLG6: a phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage.

作者信息

Jespers L S, De Keyser A, Stanssens P E

机构信息

Corvas International N.V., 9000 Gent, Belgium.

出版信息

Gene. 1996 Sep 16;173(2):179-81. doi: 10.1016/0378-1119(96)00217-x.

DOI:10.1016/0378-1119(96)00217-x

PMID:8964495

Abstract

We describe a vector, lambdaZLG6, combining the high efficiency of cDNA library cloning in bacteriophage lambda with filamentous phage display of cDNA-encoded products. The cDNAs are expressed as fusions to the 3' end of M13 gene VI. The lambdaZLG library is converted to a pZLG6-cDNA phagemid library by in vivo mass excision. Helper phage infection generates a library of phagemid particles displaying the cDNA-encoded products and containing the corresponding nucleotide sequences within.

摘要

我们描述了一种载体λZLG6，它将噬菌体λ中cDNA文库克隆的高效率与cDNA编码产物的丝状噬菌体展示相结合。cDNA作为与M13基因VI 3'端的融合蛋白表达。通过体内大规模切除，将λZLG文库转化为pZLG6 - cDNA噬菌粒文库。辅助噬菌体感染产生一个噬菌粒颗粒文库，展示cDNA编码产物并在其中包含相应的核苷酸序列。