Kijima-Tanaka M, Tamura Y, Suzuki S, Nagamine N, Nakamura M
National Veterinary Assay Laboratory, Tokyo, Japan.
J Med Microbiol. 1994 Jan;40(1):70-5. doi: 10.1099/00222615-40-1-70.
Polyclonal rabbit anti-idiotypic (anti-Id) antibodies against two monoclonal antibodies (MAbs) specific for the flagella of Clostridium chauvoei were produced, purified and characterised. Lack of cross-reactivity with heterologous MAbs indicated that the anti-Id antibodies were highly specific. The surface-exposed epitopes of the flagellar filament recognised with protective MAb were further distinguished by the anti-Id antibodies. Moreover, each anti-Id antibody inhibited the binding of its related MAb to flagellar antigens in a competitive enzyme-linked immunosorbent assay, suggesting that the anti-Id antibodies bore an internal image of the flagellar antigens. The survival rate of mice was increased to nearly twice that of controls by immunisation with anti-Id 41, which had been produced with a protective MAb; in contrast, anti-Id 114, produced with a non-protective MAb, failed to immunise. The results suggest that an anti-Id antibody containing an internal image of C. chauvoei flagella might be used as a vaccine.
制备、纯化并鉴定了针对产气荚膜梭菌鞭毛特异性的两种单克隆抗体（MAb）的多克隆兔抗独特型（抗Id）抗体。与异源单克隆抗体缺乏交叉反应表明抗Id抗体具有高度特异性。抗Id抗体进一步区分了与保护性单克隆抗体识别的鞭毛丝表面暴露表位。此外，在竞争性酶联免疫吸附试验中，每种抗Id抗体均抑制其相关单克隆抗体与鞭毛抗原的结合，表明抗Id抗体具有鞭毛抗原的内影像。用由保护性单克隆抗体制备的抗Id 41免疫小鼠，其存活率提高到近对照组的两倍；相比之下，由非保护性单克隆抗体制备的抗Id 114未能起到免疫作用。结果表明，含有产气荚膜梭菌鞭毛内影像的抗Id抗体可用作疫苗。
