Percival D A, Shuttleworth A D, Williamson E D, Kelly D C
Defence Microbiology Division, Chemical Defence Establishment, Porton Down, Salisbury, Wiltshire, United Kingdom.
Infect Immun. 1990 Aug;58(8):2487-92. doi: 10.1128/iai.58.8.2487-2492.1990.
A monoclonal antibody (BALB/c mouse) with specificity for a neutralizing epitope on the epsilon-toxin produced by Clostridium perfringens type D was used to raise anti-idiotypic antibodies (anti-Id) in different strains of mice and rabbits. These were purified and used in cross-immunization studies to induce anti-(anti-idiotype). All strains of mice and rabbits immunized with BALB/c-derived anti-Id showed a high-titer antibody response directed towards the active site of the toxin. This protected the animals against toxin challenge and against an oral dose of the vegetative organisms. Animals immunized with other anti-Id preparations showed no specific antibody response and were not protected. Guinea pig peritoneal macrophages have a cell surface receptor for the toxin, and incubation of these cells with BALB/c anti-Id allowed them to survive toxin challenge, indicating that occupation of the receptors by the anti-Id prevented binding by the toxin. In conclusion, we have shown that an internal-image anti-Id preparation will induce protective immunity in syngeneic and xenogeneic animals and furthermore that immunity to a single epitope on the exotoxin is sufficient to protect against the toxin and clinical sequelae evoked by the disease-causing organism itself.
一种对产气荚膜梭菌D型产生的ε毒素的中和表位具有特异性的单克隆抗体（BALB/c小鼠）被用于在不同品系的小鼠和兔子中产生抗独特型抗体（抗Id）。这些抗体被纯化并用于交叉免疫研究以诱导抗（抗独特型）抗体。所有用源自BALB/c的抗Id免疫的小鼠和兔子品系都表现出针对毒素活性位点的高滴度抗体反应。这保护动物免受毒素攻击以及口服剂量的营养体生物的侵害。用其他抗Id制剂免疫的动物未表现出特异性抗体反应且未得到保护。豚鼠腹腔巨噬细胞具有该毒素的细胞表面受体，将这些细胞与BALB/c抗Id一起孵育可使其在毒素攻击下存活，这表明抗Id占据受体可防止毒素结合。总之，我们已表明一种内影像抗Id制剂将在同基因和异种动物中诱导保护性免疫，而且对一种外毒素上单个表位的免疫足以保护动物免受毒素以及致病生物体本身引发的临床后遗症的侵害。
