Kempin S A, Mandel M A, Yanofsky M F
University of California, Department of Biology, San Diego, La Jolla 92093-0116.
Plant Physiol. 1993 Dec;103(4):1041-6. doi: 10.1104/pp.103.4.1041.
Mutations in the AGAMOUS (AG) gene of Arabidopsis thaliana result in the conversion of reproductive organs, stamens and carpels, into perianth organs, sepals and petals. We have isolated and characterized the putative AG gene from Nicotiana tabacum, NAG1, whose deduced protein product shares 73% identical amino acid residues with the Arabidopsis AG gene product. RNA tissue in situ hybridizations show that NAG1 RNA accumulates early in tobacco flower development in the region of the floral meristem that will later give rise to stamens and carpels. Ectopic expression of NAG1 in transgenic tobacco plants results in a conversion of sepals and petals into carpels and stamens, respectively, indicating that NAG1 is sufficient to convert perianth into reproductive floral organs.
拟南芥AGAMOUS(AG)基因的突变会导致生殖器官,即雄蕊和心皮,转变为花被器官,即萼片和花瓣。我们已经从烟草中分离并鉴定了假定的AG基因NAG1,其推导的蛋白质产物与拟南芥AG基因产物有73%的相同氨基酸残基。RNA组织原位杂交表明,NAG1 RNA在烟草花发育早期在花分生组织区域积累,该区域随后将发育出雄蕊和心皮。NAG1在转基因烟草植株中的异位表达分别导致萼片和花瓣转变为心皮和雄蕊,这表明NAG1足以将花被转变为生殖花器官。