Pnueli L, Hareven D, Rounsley S D, Yanofsky M F, Lifschitz E
Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
Plant Cell. 1994 Feb;6(2):163-73. doi: 10.1105/tpc.6.2.163.
To understand the details of the homeotic systems that govern flower development in tomato and to establish the ground rules for the judicious manipulation of this floral system, we have isolated the tomato AGAMOUS gene, designated TAG1, and examined its developmental role in antisense and sense transgenic plants. The AGAMOUS gene of Arabidopsis is necessary for the proper development of stamens and carpels and the prevention of indeterminate growth of the floral meristem. Early in flower development, TAG1 RNA accumulates uniformly in the cells fated to differentiate into stamens and carpels and later becomes restricted to specific cell types within these organs. Transgenic plants that express TAG1 antisense RNA display homeotic conversion of third whorl stamens into petaloid organs and the replacement of fourth whorl carpels with pseudocarpels bearing indeterminate floral meristems with nested perianth flowers. A complementary phenotype was observed in transgenic plants expressing the TAG1 sense RNA in that first whorl sepals were converted into mature pericarpic leaves and sterile stamens replaced the second whorl petals.
为了解调控番茄花发育的同源异型系统的细节，并为明智地操控该花系统制定基本规则，我们分离出了番茄AGAMOUS基因，命名为TAG1，并研究了其在反义及正义转基因植株中的发育作用。拟南芥的AGAMOUS基因对于雄蕊和心皮的正常发育以及防止花分生组织的无限生长是必需的。在花发育早期，TAG1 RNA在注定分化为雄蕊和心皮的细胞中均匀积累，随后局限于这些器官内的特定细胞类型。表达TAG1反义RNA的转基因植株表现出第三轮雄蕊向花瓣状器官的同源异型转化，以及第四轮心皮被带有无限花分生组织和嵌套花被花的假果所取代。在表达TAG1正义RNA的转基因植株中观察到了互补表型，即第一轮萼片转化为成熟的果皮状叶片，不育雄蕊取代了第二轮花瓣。
