Hansen G, Estruch J J, Sommer H, Spena A
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Mol Gen Genet. 1993 May;239(1-2):310-2. doi: 10.1007/BF00281633.
We report the cloning and DNA sequence of a cDNA from Nicotiana tabacum, NTGLO, as well as the pattern of expression of the NTGLO gene in wild-type tobacco plants. The NTGLO cDNA encodes a protein of 209 amino acids, which shows 73% identity with the GLO protein encoded by the GLO gene of Antirrhinum majus, a homeotic gene involved in the genetic control of flower development. Northern blot analysis shows that the NTGLO gene is expressed mainly in floral organs and, within the flower, expression is restricted to petals and stamens. The NTGLO gene most probably represents a true homologue of the GLO gene because: i) the MADS boxes, of the two genes are highly homologous (56 out of 58 amino acids are identical): ii) at the carboxy-terminal a block of 19 amino acids is perfectly conserved between the NTGLO and GLO proteins and iii) their expression patterns in floral organs are identical.
我们报道了来自烟草（Nicotiana tabacum）的一个cDNA（NTGLO）的克隆及DNA序列，以及NTGLO基因在野生型烟草植株中的表达模式。NTGLO cDNA编码一个由209个氨基酸组成的蛋白质，该蛋白质与金鱼草（Antirrhinum majus）GLO基因编码的GLO蛋白有73%的同一性，金鱼草的GLO基因是一个参与花发育遗传控制的同源异型基因。Northern杂交分析表明，NTGLO基因主要在花器官中表达，并且在花内，表达仅限于花瓣和雄蕊。NTGLO基因很可能代表了GLO基因的一个真正同源物，因为：i）这两个基因的MADS框高度同源（58个氨基酸中有56个相同）；ii）在羧基末端，NTGLO和GLO蛋白之间有19个氨基酸组成的一个区域完全保守；iii）它们在花器官中的表达模式相同。
