Springer B, Kirschner P, Rost-Meyer G, Schröder K H, Kroppenstedt R M, Böttger E C
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
J Clin Microbiol. 1993 Dec;31(12):3083-9. doi: 10.1128/jcm.31.12.3083-3089.1993.
Mycobacterium-like organisms, isolates 2081/92 and 4185/92, were recovered from a lymph node of a child with chronic lymphadenitis. The growth characteristics, acid-fastness, and mycolic acids of the isolate were consistent with those for Mycobacterium species. The isolates were biochemically distinct from described Mycobacterium species, although they most closely resembled M. scrofulaceum. Comparative 16S rDNA sequencing showed that these isolates represent a new slow-growing Mycobacterium species which is named M. interjectum. Our results demonstrate the importance of 16S rDNA sequencing for recognizing the diversity of species within the genus Mycobacterium.
类分枝杆菌，菌株2081/92和4185/92，从一名患有慢性淋巴结炎儿童的淋巴结中分离得到。该菌株的生长特性、抗酸性和分枝菌酸与分枝杆菌属的特性一致。这些菌株在生化特性上与已描述的分枝杆菌种类不同，尽管它们与瘰疬分枝杆菌最为相似。比较16S rDNA测序表明，这些菌株代表一种新的缓慢生长的分枝杆菌物种，命名为插入分枝杆菌。我们的结果证明了16S rDNA测序对于识别分枝杆菌属内物种多样性的重要性。
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