Haas W H, Butler W R, Kirschner P, Plikaytis B B, Coyle M B, Amthor B, Steigerwalt A G, Brenner D J, Salfinger M, Crawford J T, Böttger E C, Bremer H J
Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany.
J Clin Microbiol. 1997 Dec;35(12):3203-9. doi: 10.1128/jcm.35.12.3203-3209.1997.
Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differentiated biochemically from described Mycobacterium species, although it most closely resembled Mycobacterium malmoense by thin-layer chromatography and high-performance liquid chromatography of mycolic acids. A striking characteristic of the isolate was its high degree of susceptibility to antituberculous drugs in vitro, including isoniazid. Direct determination of the 16S rRNA gene sequence revealed a unique sequence and positioned the strain phylogenetically on a branch separate from M. malmoense within a group of slowly growing mycobacteria that show a high degree of similarity to M. simiae at the 16S rRNA gene level. Despite 99.6% sequence identity with M. simiae at the 16S rRNA gene level, DNA-DNA hybridization studies (hydroxyapatite method) demonstrated DNA relatedness of less than 40%. We conclude that this organism is a new species for which we propose the name M. heidelbergense. A culture of the type strain, strain 2554/91, has been deposited in the American Type Culture Collection as strain ATCC 51253.
非结核分枝杆菌性淋巴结炎在免疫功能正常的幼儿中呈现出日益严重的临床问题。从一名复发性颈淋巴结炎患儿的淋巴组织中两次分离出一种生长缓慢、非产色的分枝杆菌（菌株2553/91和2554/91）。尽管通过薄层色谱法和分枝菌酸的高效液相色谱法，该菌与马尔默分枝杆菌最为相似，但在生化特性上可与已描述的分枝杆菌菌种相区分。该分离株的一个显著特征是其在体外对包括异烟肼在内的抗结核药物高度敏感。对16S rRNA基因序列的直接测定揭示了一个独特的序列，并在系统发育上将该菌株定位在一组生长缓慢的分枝杆菌中与马尔默分枝杆菌分开的一个分支上，这些分枝杆菌在16S rRNA基因水平上与猿分枝杆菌具有高度相似性。尽管在16S rRNA基因水平上与猿分枝杆菌的序列同一性为99.6%，但DNA-DNA杂交研究（羟基磷灰石法）显示其DNA相关性低于40%。我们得出结论，该微生物是一个新物种，我们提议将其命名为海德堡分枝杆菌。模式菌株2554/91的培养物已作为菌株ATCC 51253保藏于美国典型培养物保藏中心。
