Di Stefano A, Maestrelli P, Roggeri A, Turato G, Calabro S, Potena A, Mapp C E, Ciaccia A, Covacev L, Fabbri L M, Saetta M
Institute of Occupational Medicine, University of Padova, Italy.
Am J Respir Crit Care Med. 1994 Mar;149(3 Pt 1):803-10. doi: 10.1164/ajrccm.149.3.7509705.
To determine whether adhesion molecules and cytokines are upregulated in the bronchial mucosa of chronic bronchitics, we obtained bronchial biopsies in 16 chronic bronchitics, in eight asymptomatic smokers, and in seven normal nonsmoking subjects. Bronchial biopsies were examined by immunohistochemistry to identify the expression of E-selectin and intercellular adhesion molecular-1 (ICAM-1) on vessels and on bronchial epithelium, and the expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), neutrophil elastase, and eosinophil cationic protein (EG-2) on cells in the submucosa. Chronic bronchitics had an increased number of E-selectin-positive vessels when compared with both asymptomatic smokers (p < 0.05) and normal subjects (p < 0.01). The numbers of ICAM-1-positive vessels, neutrophils, and IL-1 beta, TNF-alpha-, and EG-2-positive cells were not significantly different in the three groups of subjects examined. When the bronchitic group was divided according to the presence or absence of airway obstruction, the increased number of E-selectin-positive vessels persisted only in bronchitics with airway obstruction, who also had an increased expression of ICAM-1 on basal epithelial cells. We concluded that in the bronchial mucosa of chronic bronchitics with airway obstruction, there is an increased expression of E-selectin on vessels and of ICAM-1 on basal epithelial cells, suggesting the involvement of these adhesion molecules in the pathogenesis of the disease.
为了确定慢性支气管炎患者支气管黏膜中黏附分子和细胞因子是否上调，我们对16例慢性支气管炎患者、8例无症状吸烟者和7例正常非吸烟者进行了支气管活检。通过免疫组织化学检查支气管活检组织，以鉴定血管和支气管上皮细胞上E-选择素和细胞间黏附分子-1（ICAM-1）的表达，以及黏膜下层细胞白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、中性粒细胞弹性蛋白酶和嗜酸性粒细胞阳离子蛋白（EG-2）的表达。与无症状吸烟者（p < 0.05）和正常受试者（p < 0.01）相比，慢性支气管炎患者E-选择素阳性血管数量增加。在三组受试对象中，ICAM-1阳性血管、中性粒细胞以及IL-1β、TNF-α和EG-2阳性细胞的数量无显著差异。根据是否存在气道阻塞将支气管炎组进行划分时，E-选择素阳性血管数量增加仅存在于有气道阻塞的支气管炎患者中，这些患者基底上皮细胞上ICAM-1的表达也增加。我们得出结论，在有气道阻塞的慢性支气管炎患者的支气管黏膜中，血管上E-选择素和基底上皮细胞上ICAM-1的表达增加，提示这些黏附分子参与了该疾病的发病机制。
