Aki T, Ono K, Paik S Y, Wada T, Jyo T, Shigeta S, Murooka Y, Oka S
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Int Arch Allergy Immunol. 1994;103(4):349-56. doi: 10.1159/000236653.
A cDNA clone encoding a new allergen from the Dermatophagoides farinae cDNA lambda gt11 library was isolated and sequenced. There was no amino acid sequence homology with other known allergens. The gene product, beta-galactosidase fusion protein, of the truncated cDNA on blot reacted with IgE in 13 of 43 sera from patients allergic to mites. The affinity-purified fusion protein had a potent ability to release histamine from washed blood cells of the mite-allergic patients. Human specific IgE eluted from the fusion protein band on blots recognized a 39-kD component on blots of a mite body extract.
从粉尘螨cDNAλgt11文库中分离并测序了一个编码新过敏原的cDNA克隆。其氨基酸序列与其他已知过敏原无同源性。截短cDNA的基因产物β-半乳糖苷酶融合蛋白，在印迹上与43例螨过敏患者血清中的13例IgE发生反应。亲和纯化的融合蛋白具有从螨过敏患者的洗涤血细胞中释放组胺的强大能力。从印迹上的融合蛋白条带洗脱的人特异性IgE识别螨体提取物印迹上的一个39-kD成分。
