Fujikawa A, Ishimaru N, Seto A, Yamada H, Aki T, Shigeta S, Wada T, Jyo T, Murooka Y, Oka S, Ono K
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Mol Immunol. 1996 Feb;33(3):311-9. doi: 10.1016/0161-5890(95)00127-1.
A new immunoreactive clone whose sequence is not homologous to that of any of the previously identified mite allergens was isolated by successive immunoscreening of a Dermatophagoides farinae cDNA library with rabbit antisera to an extract of the house dust mite and IgE in pooled sera from patients allergic to mites. Rabbit antibodies specific for the recombinant protein recognized a 177 kD protein in a mite body extract. This immunoreactive protein was located in the circumferential tissues of esophagus, gut and the other internal organs in mites. The reaction of human IgE to the purified natural antigen was inhibited competitively to 30% by the recombinant antigen. In terms of the frequency and the intensity of response to specific IgE in sera from asthmatic patients, the natural protein was similar to Der f2, while the recombinant protein was slightly less allergenic by these criteria. We conclude that the natural protein from the house dust mite, D. farinae, is an important allergen.
通过用兔抗屋尘螨提取物血清和对螨过敏患者混合血清中的IgE对粉尘螨cDNA文库进行连续免疫筛选，分离出了一个新的免疫反应性克隆，其序列与任何先前鉴定的螨过敏原均不同源。针对重组蛋白的兔抗体在螨体提取物中识别出一种177 kD的蛋白。这种免疫反应性蛋白位于螨的食管、肠道和其他内脏器官的周边组织中。重组抗原可竞争性抑制人IgE对纯化天然抗原的反应达30%。就哮喘患者血清中对特异性IgE的反应频率和强度而言，天然蛋白与Der f2相似，而根据这些标准，重组蛋白的致敏性略低。我们得出结论，来自屋尘螨（粉尘螨）的天然蛋白是一种重要的过敏原。
