Aki T, Kodama T, Fujikawa A, Miura K, Shigeta S, Wada T, Jyo T, Murooka Y, Oka S, Ono K
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Japan.
J Allergy Clin Immunol. 1995 Jul;96(1):74-83. doi: 10.1016/s0091-6749(95)70035-8.
Two predominant mite species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, are responsible for the immediate hypersensitivity reaction. Although a large number of antigens and allergens was detected in a whole mite culture extract, only the major allergens of Der I and II groups have been extensively studied. Much information on other important allergens remains to be accumulated.
A new allergen complementary DNA (cDNA) clone was isolated from a D. farinae protein cDNA library. The expression product of the cDNA and native tropomyosin from D. farinae were purified, characterized, and evaluated.
An amino acid sequence deduced from the cDNA clone revealed significant homology with tropomyosins conserved in a wide range of animals. The amino acid sequences of two fragments obtained by degradation of the purified protein with cyanogen bromide coincided completely with the corresponding portions of the deduced amino acid sequence. The native tropomyosin reacted with specific IgE in the 31 sera tested at a high frequency (80.6%), comparable to that of Der f I (90.3%) and Der f II (74.2%).
The cloned cDNA encodes tropomyosin. The high binding response of specific IgE antibodies to recombinant and native tropomyosins demonstrates that mite tropomyosin is an additional important allergen in house dust mite.
两种主要的螨类，即粉尘螨和屋尘螨，是引起速发型超敏反应的原因。尽管在全螨培养提取物中检测到大量抗原和过敏原，但仅对Der I和II组的主要过敏原进行了广泛研究。关于其他重要过敏原的许多信息仍有待积累。
从粉尘螨蛋白质cDNA文库中分离出一个新的过敏原互补DNA（cDNA）克隆。对该cDNA的表达产物和粉尘螨的天然原肌球蛋白进行了纯化、表征和评估。
从cDNA克隆推导的氨基酸序列显示与广泛动物中保守的原肌球蛋白具有显著同源性。用溴化氰降解纯化蛋白得到的两个片段的氨基酸序列与推导的氨基酸序列的相应部分完全一致。在测试的31份血清中，天然原肌球蛋白与特异性IgE的反应频率很高（80.6%），与Der f I（90.3%）和Der f II（74.2%）相当。
克隆的cDNA编码原肌球蛋白。特异性IgE抗体对重组和天然原肌球蛋白的高结合反应表明，螨原肌球蛋白是屋尘螨中的另一种重要过敏原。
