Kocharova N A, Knirel Y A, Shashkov A S, Nifant'ev N E, Kochetkov N K, Varbanets L D, Moskalenko N V, Brovarskaya O S, Muras V A, Young J M
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Carbohydr Res. 1993 Dec 28;250(2):275-87. doi: 10.1016/0008-6215(93)84006-r.
The structures of the O-antigenic polysaccharide chains of lipopolysaccharides of a number of Pseudomonas solanacearum strains were elucidated mainly with the help of methylation analysis and 13C NMR spectroscopy, including a computer-assisted 13C NMR-based analysis. Six structurally distinct but related polysaccharides were identified. They have a backbone which is built up of three L-rhamnopyranosyl residues and one 2-acetamido-2-deoxy-D-glucopyranosyl residue, and is unsubstituted or substituted with a residue of L-xylopyranose or L-rhamnopyranose as a monosaccharide side chain. The lipopolysaccharides of most of the strains contain polysaccharide chains consisting of at least two structurally different types of repeating units. Three of the polysaccharides are common to more than one strain.
主要借助甲基化分析和13C核磁共振光谱法，包括基于计算机辅助的13C核磁共振分析，阐明了多种青枯雷尔氏菌菌株脂多糖的O抗原多糖链结构。鉴定出六种结构不同但相关的多糖。它们具有由三个L-吡喃鼠李糖残基和一个2-乙酰氨基-2-脱氧-D-吡喃葡萄糖残基组成的主链，该主链未被取代或被L-吡喃木糖或L-吡喃鼠李糖残基作为单糖侧链取代。大多数菌株的脂多糖含有由至少两种结构不同类型的重复单元组成的多糖链。其中三种多糖为多个菌株所共有。
