Meyer J, Whitcomb L, Collins D
Department of Pharmaceutics and Drug Delivery, Amgen, Inc., Thousand Oaks, CA 91320.
Biochem Biophys Res Commun. 1994 Mar 15;199(2):433-8. doi: 10.1006/bbrc.1994.1247.
A highly efficient method for the liposome encapsulation of granulocyte colony stimulating factor (rhG-CSF) was developed. The method was found to be gentle and led to no protein aggregation, denaturation or loss of protein activity. The liposomes obtained were judged to be oligolamellar based on a comparison of the actual with the theoretical trapped volumes. Slow release of encapsulated material from the liposomes was demonstrated both in vitro (90% serum, 37 degrees C) and in vivo after subcutaneous injection.
开发了一种用于粒细胞集落刺激因子（rhG-CSF）脂质体包封的高效方法。发现该方法温和，不会导致蛋白质聚集、变性或蛋白质活性丧失。根据实际截留体积与理论截留体积的比较，判断所得脂质体为寡层脂质体。在体外（90%血清，37℃）和皮下注射后的体内均证明了包封材料从脂质体中的缓慢释放。
