Dermon C R, Stamatakis A
Department of Biology, University of Crete, Greece.
Neuroreport. 1994 Oct 3;5(15):1941-5. doi: 10.1097/00001756-199410000-00025.
The present study aimed to define the distribution of NADPH-diaphorase (NADPH-d) positive cells and fibers in the developing chick cerebellum. From embryonic day 9 (E9) until day E15 NADPH-d positive ascending axons, originating from pontine nuclei, targeted selectively the developing folia. Cerebellar cortex exhibited weak NADPH-d activity until day E15, when NADPH-d negative granule cells of external granular layer began inward migration. From that embryonic age, differentiated granule, Golgi II type cells and glomeruli of the internal granular layer exhibited gradually increasing diaphorase activity. In the molecular layer diffuse activity increased, possibly due to the development of the parallel fibers. Transient NADPH-d activity appeared on Purkinje somata (PC) at day E17 and by posthatching day 1 all PC were devoid of diaphorase activity. Our data support the hypothesis that neurons producing nitric oxide (NO) are involved in the activity dependent developmental processes for the establishment of connections and normal cytoarchitecture.
本研究旨在确定发育中的雏鸡小脑内烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）阳性细胞和纤维的分布。从胚胎第9天（E9）到E15天，源自脑桥核的NADPH-d阳性上升轴突选择性地靶向发育中的小叶。直到E15天，小脑皮质的NADPH-d活性较弱，此时外颗粒层的NADPH-d阴性颗粒细胞开始向内迁移。从那个胚胎时期开始，内颗粒层分化的颗粒细胞、高尔基II型细胞和肾小球的黄递酶活性逐渐增加。在分子层，弥漫性活性增加，可能是由于平行纤维的发育。E17天时，浦肯野细胞胞体（PC）出现短暂的NADPH-d活性，到孵化后第1天，所有PC均无黄递酶活性。我们的数据支持这样的假设，即产生一氧化氮（NO）的神经元参与了依赖活动的发育过程，以建立连接和正常的细胞结构。
