Tammi R, Rönkkö S, Agren U M, Tammi M
Department of Anatomy, University of Kuopio, Finland.
J Histochem Cytochem. 1994 Nov;42(11):1479-86. doi: 10.1177/42.11.7523491.
To study the expression of hyaluronan in male reproductive organs and the origin of seminal plasma hyaluronan, we stained various parts of the bull reproductive tract for hyaluronan using a biotinylated probe derived from cartilage proteoglycan (bHABC). The potential loss of hyaluronan during tissue processing was checked with a novel technique by blotting frozen tissue sections on nitrocellulose and staining the blots with bHABC. In the same tissues the CD44 receptor was visualized by Hermes 1 antibody. The testes showed only traces of hyaluronan, whereas both the epithelium and the connective tissue of seminal vesicle, prostate, Cowper's gland, and epididymis were positive in bHABC staining. Hyaluronan was localized on the basolateral surfaces of these epithelial cells. The secretions inside the seminal vesicle and in the ducts of prostate and Cowper's gland were HA-positive, whereas the luminal contents of seminiferous tubules and epididymis were unstained both in paraffin sections and in the in situ blocks. The data indicate that hyaluronan in seminal plasma originates from the accessory sex glands. The co-localization of CD44 with hyaluronan in the basolateral surfaces of the accessory gland epithelia and its absence from other epithelia with little or no hyaluronan supports its role as a hyaluronan receptor.
为研究透明质酸在雄性生殖器官中的表达及精浆透明质酸的来源，我们使用源自软骨蛋白聚糖的生物素化探针（bHABC）对公牛生殖道的各个部位进行透明质酸染色。通过一种新技术检查组织处理过程中透明质酸的潜在损失，即将冷冻组织切片印迹在硝酸纤维素膜上，并用bHABC对印迹进行染色。在相同组织中，用Hermes 1抗体观察CD44受体。睾丸仅显示微量透明质酸，而精囊、前列腺、尿道球腺和附睾的上皮及结缔组织在bHABC染色中均呈阳性。透明质酸定位于这些上皮细胞的基底外侧表面。精囊内以及前列腺和尿道球腺导管内的分泌物呈HA阳性，而在石蜡切片和原位组织块中，曲细精管和附睾的管腔内容物均未染色。数据表明精浆中的透明质酸源自附属性腺。CD44与透明质酸在附属腺上皮基底外侧表面的共定位以及在其他几乎没有或没有透明质酸的上皮中的缺失，支持了其作为透明质酸受体的作用。
