Power W, Neylan D, Collum L
Department of Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland.
Doc Ophthalmol. 1993;84(4):351-63. doi: 10.1007/BF01215449.
A system for culturing human lens epithelial cells in the laboratory was developed. The morphological appearances of the cells was studied using phase contrast, scanning and transmission electron microscopy. Cell marker studies using monoclonal antibodies to cytokeratin, vimentin and epithelial membrane antigen were also performed. There was a marked increase in cell size as a function of time in culture. After 3 to 4 weeks cells showed early signs of ageing. By 6 to 8 weeks the majority of the cells had become very irregular in shape and demonstrated irregularities of the plasma membrane and intra-cytoplasmic vacuole formation. The cells stained strongly for vimentin and epithelial membrane antigen. Staining with cytokeratin was somewhat weaker. This culture technique provides us with a suitable model for studying the growth behavior of these cells.
开发了一种在实验室培养人晶状体上皮细胞的系统。使用相差显微镜、扫描电子显微镜和透射电子显微镜研究了细胞的形态外观。还进行了使用针对细胞角蛋白、波形蛋白和上皮膜抗原的单克隆抗体的细胞标志物研究。随着培养时间的推移，细胞大小显著增加。3至4周后，细胞显示出早期衰老迹象。到6至8周时，大多数细胞的形状变得非常不规则，质膜出现异常，胞质内形成空泡。细胞对波形蛋白和上皮膜抗原染色强烈。细胞角蛋白染色稍弱。这种培养技术为我们研究这些细胞的生长行为提供了一个合适的模型。
