Kramer K, Hicks D G, Palis J, Rosier R N, Oppenheimer J, Fallon M D, Cohen H J
Strong Memorial Hospital, Rochester, New York.
Cancer. 1993 May 15;71(10):2977-82. doi: 10.1002/1097-0142(19930515)71:10<2977::aid-cncr2820711015>3.0.co;2-u.
The combination of a primary osteosarcoma of bone with a second carcinomatous cell type has been recognized, although immunohistochemical studies currently have not been performed in an attempt to understand the histogenesis of such a tumor.
In this report, the authors performed immunohistochemical studies on a primary osseous carcinosarcoma. Using a biotin-streptavidin peroxidase conjugate technique, the expression of keratin, epithelial membrane antigen, and vimentin was analyzed.
The epithelial cells expressed cytokeratin and epithelial membrane antigen but did not express vimentin. The mesenchymal cells strongly expressed vimentin, and only rare cells expressed cytokeratin.
The clinical, morphologic, and immunophenotypic data in this instance strongly suggest divergent differentiation of a primitive multipotential uncommitted stem cell in a primary osseous tumor.
骨原发性骨肉瘤与第二种癌性细胞类型的组合已得到认可，尽管目前尚未进行免疫组织化学研究以试图了解此类肿瘤的组织发生。
在本报告中，作者对原发性骨癌肉瘤进行了免疫组织化学研究。采用生物素 - 链霉亲和素过氧化物酶共轭技术，分析了角蛋白、上皮膜抗原和波形蛋白的表达。
上皮细胞表达细胞角蛋白和上皮膜抗原，但不表达波形蛋白。间充质细胞强烈表达波形蛋白，只有极少数细胞表达细胞角蛋白。
该病例的临床、形态学和免疫表型数据强烈提示原发性骨肿瘤中原始多能未定向干细胞的分化。
