Jacob T J
Department of Pharmacology, St George's Hospital Medical School, London, U.K.
Exp Eye Res. 1987 Jul;45(1):93-104. doi: 10.1016/s0014-4835(87)80081-7.
Human lens epithelial (HLE) cells were explanted into medium containing different concentrations of foetal calf serum (FCS) and serum substitute. The proliferation of cells was measured as a function of time in culture and the growth parameters (growth rate and proliferative capacity) were determined by the application of the Gompertz growth function. Human lens epithelial cells have a limited growth capacity in culture of five to seven population doublings and this decreases with age. The population doubling level (PDL) was determined empirically and from fits of the Gompertz equation. The values of the PDL determined experimentally and theoretically are compared for a series of different culture conditions. Exposure of the cells to raised levels of FCS caused a reduction in proliferative capacity; the PDL decreased from 6.3 to 5.5 when the serum was raised to 50% although the growth rate increased. Serum substitute caused a decrease in the growth rate without changing the proliferative capacity and also altered the cellular morphology. It is hoped that the method described will have applications to the study of posterior capsule opacification following extracapsular cataract extraction and phacoemulsification and will aid in the development of preventive therapies.
将人晶状体上皮（HLE）细胞接种到含有不同浓度胎牛血清（FCS）和血清替代品的培养基中。根据培养时间测定细胞增殖情况，并通过应用Gompertz生长函数确定生长参数（生长速率和增殖能力）。人晶状体上皮细胞在培养中的生长能力有限，约为五到七个群体倍增，且随着年龄增长而下降。群体倍增水平（PDL）通过经验方法并根据Gompertz方程拟合来确定。比较了一系列不同培养条件下实验测定和理论计算得到的PDL值。将细胞暴露于高水平的FCS会导致增殖能力下降；当血清浓度提高到50%时，PDL从6.3降至5.5，尽管生长速率有所增加。血清替代品会导致生长速率下降，但不改变增殖能力，同时也会改变细胞形态。希望所描述的方法能够应用于囊外白内障摘除和超声乳化术后后囊膜混浊的研究，并有助于开发预防性治疗方法。
