Nikolov C, Detcheva Z
Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(5):633-8.
The authors tested preserving properties of three concentrations of dimethylsulphoxide (15%, 10% and 7.5%) in preservation of rat bone marrow cells at -150 degrees C. Cells of rat bone marrow were frozen at 1 degree C/min to -20 degrees C, 5 degrees C/min to -80 degrees C and then placed directly at -150 degrees C and held at such temperature for 6 months. Vitality of cells was checked monthly for a period of 6 months by means of several vitality tests with dyes (eosin and trypane blue), autoradiography and erythrophagocytosis. It was found that cells capable of cleavage could be equally preserved at such low temperature with all the three DMSO concentrations while mature cells (granulocytes, reticular cells) revealed considerably higher erythrophagocytic activity when preserved at 15% DMSO and lower activity at 10% and 7.5% DMSO.
作者测试了三种浓度（15%、10%和7.5%）的二甲基亚砜在-150℃保存大鼠骨髓细胞时的保存特性。大鼠骨髓细胞先以1℃/分钟的速度冷冻至-20℃，再以5℃/分钟的速度冷冻至-80℃，然后直接置于-150℃并在此温度下保存6个月。在6个月的时间里，每月通过几种用染料（伊红和台盼蓝）进行的活力测试、放射自显影和红细胞吞噬作用来检查细胞活力。结果发现，三种二甲基亚砜浓度在如此低温下均能同等程度地保存可分裂细胞，而成熟细胞（粒细胞、网状细胞）在15%二甲基亚砜保存时显示出明显更高的红细胞吞噬活性，在10%和7.5%二甲基亚砜保存时活性较低。
