Grossman A B, Rossmanith W G, Kabigting E B, Cadd G, Clifton D, Steiner R A
Department of Endocrinology, St. Bartholomew's Hospital, London, UK.
J Endocrinol. 1994 Feb;140(2):R5-8. doi: 10.1677/joe.0.140r005.
Using probes for rat neural nitric oxide synthase (NOS) mRNA and GnRH mRNA, we performed in situ hybridization to survey NOS mRNA distribution within the hypothalamus of the male and female rat and sought evidence for its expression in GnRH neurons. The NOS cRNA probe was radiolabelled with 35S, and a digoxigenin-labeled rat GnRH cRNA probe was used for double-label studies. NOS mRNA was localized in discrete hypothalamic areas, in grain clusters suggestive of individual neurons. NOS mRNA-positive cells were located mainly in the supraoptic and paraventricular nucleus, particularly overlying the magnocellular division. Rostrally, cells expressing NOS mRNA were especially prominent in the diagonal band of Broca, in a distribution very similar to GnRH neurons. Nevertheless, only one of 370 cells labeled for GnRH mRNA appeared to be positive for NOS mRNA. We conclude that NOS mRNA is located prominently in regions where CRH, AVP and oxytocin cells are located. NOS mRNA-positive cells are located in close proximity to GnRH neurons, but rarely do such neurons express NOS mRNA.
我们使用大鼠神经型一氧化氮合酶（NOS）mRNA和促性腺激素释放激素（GnRH）mRNA的探针，进行原位杂交以研究雄性和雌性大鼠下丘脑中NOS mRNA的分布，并寻找其在GnRH神经元中表达的证据。NOS cRNA探针用35S进行放射性标记，地高辛标记的大鼠GnRH cRNA探针用于双重标记研究。NOS mRNA定位于下丘脑的离散区域，呈颗粒簇状，提示单个神经元。NOS mRNA阳性细胞主要位于视上核和室旁核，特别是在大细胞部上方。在嘴侧，表达NOS mRNA的细胞在布洛卡斜带中尤为突出，其分布与GnRH神经元非常相似。然而，在370个标记为GnRH mRNA的细胞中，只有一个似乎对NOS mRNA呈阳性。我们得出结论，NOS mRNA主要位于促肾上腺皮质激素释放激素（CRH）、精氨酸加压素（AVP）和催产素细胞所在的区域。NOS mRNA阳性细胞位于GnRH神经元附近，但这些神经元很少表达NOS mRNA。
