Kotani T, Aratake Y, Kondo S, Tamura K, Ohtaki S
Department of Laboratory Medicine, Miyazaki Medical College, Kiyotake, Japan.
Leuk Res. 1994 Apr;18(4):305-10. doi: 10.1016/0145-2126(94)90034-5.
The expression of Fas antigen was analyzed in the peripheral blood mononuclear cells of 12 patients with adult T-cell leukemia (ATL) by flow cytometry. The induction of apoptosis in these cells by adding an anti-Fas antibody and the expression of activated T-cell surface antigens, CD25 and CD26, were also studied. It appears that the cells in ATL expressed a significantly larger number of Fas antigens than those in normal subjects (p < 0.01) and their fluorescent intensity was also shown to be much stronger in ATL (p < 0.01). The large number of ATL cells showed apoptosis in a short-term culture in the presence of the anti-Fas antibody. There was no difference in the expression of Fas antigen among ATL cells with different phenotypes of CD4+/CD8-, CD4-/CD8- and CD4+/CD8+ as well as with clinical subtypes of ATL. Interestingly, the expression of Fas and CD26 antigens showed a negative correlation (p < 0.01, r = 0.78). The strong expression of functional Fas antigen in ATL leads to the impression that anti-Fas antibody could be one of the treatment modalities for ATL which is known to be a very difficult disease to cope with.
通过流式细胞术分析了12例成人T细胞白血病（ATL）患者外周血单个核细胞中Fas抗原的表达情况。还研究了添加抗Fas抗体对这些细胞凋亡的诱导作用以及活化T细胞表面抗原CD25和CD26的表达情况。结果显示，ATL患者细胞表达的Fas抗原数量明显多于正常受试者（p < 0.01），并且其荧光强度在ATL中也更强（p < 0.01）。在抗Fas抗体存在的短期培养中，大量ATL细胞出现凋亡。不同CD4+/CD8-、CD4-/CD8-和CD4+/CD8+表型的ATL细胞以及不同临床亚型的ATL细胞中Fas抗原的表达没有差异。有趣的是，Fas和CD26抗原的表达呈负相关（p < 0.01，r = 0.78）。ATL中功能性Fas抗原的强表达使人认为抗Fas抗体可能是ATL的治疗方式之一，而ATL是一种已知很难应对的疾病。
