离子强度对天然及乙酰化核糖核酸酶活性的影响
Effect of ionic strength on the activity of native and acetylated ribonuclease.
作者信息
Wieczorek M
出版信息
Acta Biochim Pol. 1978;25(4):391-7.
PMID:751364
Abstract
Acetylation of free amino groups in RNAase abolishes the stimulating effect of ionic strength on the enzyme activity. Distinct differences in the effect of ionic strength on catalytic function of native and acetylated RNAase are observed only with the natural substrate, RNA. In the presence of synthetic substrates, cyclic cytidine 2':3'-phosphate and cytidyl(3'-5')adenosine phosphate, this effect was not observed.
摘要
核糖核酸酶中游离氨基的乙酰化消除了离子强度对酶活性的刺激作用。仅在天然底物RNA存在时，才观察到离子强度对天然核糖核酸酶和乙酰化核糖核酸酶催化功能的影响存在明显差异。在合成底物2':3'-环磷酸胞苷和胞苷（3'-5'）磷酸腺苷存在的情况下，未观察到这种影响。
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