Dignass A U, Tsunekawa S, Podolsky D K
Department of Medicine, Massachusetts General Hospital, Boston.
Gastroenterology. 1994 May;106(5):1254-62. doi: 10.1016/0016-5085(94)90017-5.
BACKGROUND/AIMS: Various peptide growth factors have been found to exert functional effects among epithelial cell populations. This study assessed the role of certain fibroblast growth factors (FGFs) (acidic FGF, basic FGF, and keratinocyte growth factor) in the regulation of intestinal epithelial cell proliferation and restitution.
Recombinant growth factors were added to subconfluent cultures of IEC-6, Caco-2, and HT-29 cell lines with subsequent assessment of [3H]-thymidine incorporation. The effects on an in vitro model of restitution were assessed by quantitation of cells migrating into standard wounds established in confluent monolayers of IEC-6 cells. Transforming growth factor beta (TGF-beta) content of growth factor-treated wounded monolayers was assessed by Northern blot and bioassay.
Acidic FGF, basic FGF, and keratinocyte growth factor caused a modest increase in proliferation of IEC-6, Caco-2, and HT-29 cell lines. Acidic FGF and basic FGF promoted intestinal epithelial cell restitution in vitro up to 10-fold, in conjunction with the enhanced expression of TGF-beta messenger RNA and protein. Promotion of IEC-6 restitution by acidic and basic FGF could be blocked by addition of immunoneutralizing anti-TGF-beta antisera.
FGFs that exert effects on fibroblast cells also exert effects on intestinal epithelial cell populations and may help promote epithelial cell restitution, the initial step of intestinal wound healing through a TGF-beta-dependent pathway.
背景/目的：已发现多种肽生长因子在上皮细胞群体中发挥功能作用。本研究评估了某些成纤维细胞生长因子（FGFs）（酸性FGF、碱性FGF和角质形成细胞生长因子）在调节肠上皮细胞增殖和修复中的作用。
将重组生长因子添加到IEC-6、Caco-2和HT-29细胞系的亚汇合培养物中，随后评估[3H] - 胸腺嘧啶核苷掺入情况。通过对迁移到IEC-6细胞汇合单层中建立的标准伤口中的细胞进行定量，评估对体外修复模型的影响。通过Northern印迹和生物测定法评估生长因子处理的受伤单层中转化生长因子β（TGF-β）的含量。
酸性FGF、碱性FGF和角质形成细胞生长因子使IEC-6、Caco-2和HT-29细胞系的增殖适度增加。酸性FGF和碱性FGF在体外促进肠上皮细胞修复达10倍，同时TGF-β信使RNA和蛋白质的表达增强。添加免疫中和抗TGF-β抗血清可阻断酸性和碱性FGF对IEC-6修复的促进作用。
对成纤维细胞发挥作用的FGFs也对肠上皮细胞群体发挥作用，并可能通过TGF-β依赖性途径促进上皮细胞修复，这是肠伤口愈合的初始步骤。
