Duraisamy G, Zuridah H, Ariffin M Y
Blood Services Centre, Hospital, Kuala Lumpur.
Med J Malaysia. 1993 Sep;48(3):313-6.
Hepatitis C virus (HCV) is the chief aetiologic agent for the parenterally transmitted Non-A, Non-B (NANB) hepatitis. This preliminary study was done to determine the prevalence of anti-HCV in the blood donor population. Blood from 3,540 donors who donated blood to the Blood Services Centre, Hospital, Kuala Lumpur, from 25th August 1991 to 13th January 1992, was tested for anti-HCV using both the Ortho and Abbott 2nd Generation ELISA test kits. ELISA positive specimens were repeated twice but no confirmatory test was done. There were 53 out of 3,540 (1.49%) blood donors who were repeatedly reactive to anti-HCV by ELISA. We plan to do further tests to confirm the results, using RIBA-2 or Abbott Neutralising test. Twenty eight out of 1,713 (1.63%) Malays, 22 out of 1,373 (1.60%) Chinese and 2 out of 393 (0.50%) Indians had antibodies to HCV. There was no significant difference in prevalence in the different age groups. The majority of donors tested were males (3,511 out of 3,540) of which 53 (1.50%) were anti-HCV positive. Only 29 females were tested and all were negative. To determine infectivity of the anti-HCV positive cases we would like to introduce testing for RNA by polymerate chain reaction (PCR). Screening all donated blood for anti-HCV will decrease, but not totally eliminate, post-transfusion hepatitis.
丙型肝炎病毒（HCV）是经肠道外传播的非甲非乙型（NANB）肝炎的主要病原体。这项初步研究旨在确定献血人群中抗-HCV的流行率。对1991年8月25日至1992年1月13日期间在吉隆坡医院血液服务中心献血的3540名献血者的血液，使用Ortho和雅培第二代酶联免疫吸附测定（ELISA）试剂盒检测抗-HCV。ELISA阳性标本重复检测两次，但未进行确证试验。3540名献血者中有53名（1.49%）经ELISA检测抗-HCV反复呈阳性。我们计划使用重组免疫印迹法-2（RIBA-2）或雅培中和试验进行进一步检测以确认结果。1713名马来人中有28名（1.63%）、1373名华人中有22名（1.60%）、393名印度人中有2名（0.50%）有抗-HCV抗体。不同年龄组的流行率无显著差异。检测的献血者大多数为男性（3540名中的3511名），其中53名（1.50%）抗-HCV阳性。仅检测了29名女性，结果均为阴性。为确定抗-HCV阳性病例的传染性，我们希望引入聚合酶链反应（PCR）检测RNA。对所有捐献血液进行抗-HCV筛查将减少但不能完全消除输血后肝炎。
