Boutin Y, Hébert J
Centre de Recherche en Inflammation et Immunologie-Rhumatologie, Le Centre de Recherche du CHUL, Sainte-Foy, Québec, Canada.
Clin Exp Immunol. 1994 May;96(2):350-5. doi: 10.1111/j.1365-2249.1994.tb06566.x.
To study the role of anti-idiotypic antibodies in the regulation of the immune response to Lol p I (the major allergenic component of rye grass pollen), we have recently generated a panel of three MoAbs directed against distinct epitopes of Lolp I and an anti-idiotypic MoAb directed against the idiotype borne by one of the anti-Lol p I MoAbs (290A-167). The effects of pretreatment with this anti-idiotypic MoAb in BALB/c mice before immunization with the antigen have been examined. The anti-idiotypic MoAb or unrelated MoAb were given weekly for 8 weeks intraperitoneally. Mice then received the antigen (2 micrograms) adsorbed with alum (2 mg) at weeks 9, 11 and 13. Serum anti-Lol p I antibodies (IgG or IgE) and specific idiotypic responses were measured. Anti-Lol p I IgG antibodies could be detected before immunization with Lol p I only in mice pretreated with anti-idiotypic MoAb. Immunization with Lol p I induced an anti-Lol p I IgG response in both groups, but this response was higher in mice that received anti-idiotypic MoAb. Similar profiles were seen for specific IgE antibodies and idiotypic responses. Surprisingly, idiotypes borne by other anti-Lol p I MoAbs (539A-6 and 348A-6) had also been enhanced after pretreatment with the anti-290A-167 MoAb. These observations suggested that the pretreatment with this anti-idiotypic MoAb modulates not only the expression of the respective idiotype, but also affects other idiotype responses.
为研究抗独特型抗体在调节对黑麦草花粉主要变应原成分Lol p I免疫反应中的作用，我们最近制备了一组三种单克隆抗体（MoAbs），它们分别针对Lolp I的不同表位，以及一种抗独特型单克隆抗体，该抗体针对抗Lol p I单克隆抗体之一（290A - 167）所携带的独特型。我们检测了用这种抗独特型单克隆抗体预处理BALB/c小鼠后，再用抗原免疫所产生的效应。抗独特型单克隆抗体或无关单克隆抗体每周腹腔注射一次，共8周。然后在第9、11和13周给小鼠注射吸附有明矾（2mg）的抗原（2微克）。检测血清抗Lol p I抗体（IgG或IgE）以及特异性独特型反应。仅在用抗独特型单克隆抗体预处理的小鼠中，在注射Lol p I之前就能检测到抗Lol p I IgG抗体。用Lol p I免疫在两组中均诱导出抗Lol p I IgG反应，但在用抗独特型单克隆抗体处理的小鼠中该反应更高。特异性IgE抗体和独特型反应也呈现类似情况。令人惊讶的是，在用抗290A - 167单克隆抗体预处理后，其他抗Lol p I单克隆抗体（539A - 6和348A - 6）所携带的独特型也增强了。这些观察结果表明，用这种抗独特型单克隆抗体预处理不仅调节了相应独特型的表达，还影响了其他独特型反应。
