von Schönfeld J, Müller M K
Department of Gastroenterology, University of Essen, Germany.
Experientia. 1994 May 15;50(5):442-6. doi: 10.1007/BF01920743.
Intravenous glucagon inhibits exocrine pancreatic secretion in vivo, but exogenous glucagon does not affect exocrine secretion in vitro. Recent work, however, suggested that endogenous glucagon may be involved in the regulation of exocrine secretion even in vitro. We therefore investigated the effects of exogenous and endogenous glucagon on exocrine secretion by the isolated perfused rat pancreas in the presence of 1.8 mM glucose. Exogenous glucagon did not affect CCK-stimulated amylase output. 20 mM arginine stimulated glucagon release, but did not affect basal enzyme secretion. CCK-stimulated amylase output, however, was significantly inhibited in the presence of arginine. This inhibitory effect of arginine on exocrine pancreatic secretion could be blocked by glucagon antibodies, but not by nonspecific gammaglobulins. Thus exogenous glucagon failed to affect exocrine pancreatic secretion in vitro, but endogenously released glucagon or a glucagon-like peptide inhibited amylase release in the isolated perfused pancreas. We conclude that glucagon or a glucagon-like peptide may be a mediator in the islet-acinar axis.
静脉注射胰高血糖素在体内可抑制胰腺外分泌，但外源性胰高血糖素在体外并不影响外分泌。然而，最近的研究表明，即使在体外，内源性胰高血糖素也可能参与外分泌的调节。因此，我们研究了在1.8 mM葡萄糖存在的情况下，外源性和内源性胰高血糖素对离体灌注大鼠胰腺外分泌的影响。外源性胰高血糖素不影响胆囊收缩素刺激的淀粉酶分泌。20 mM精氨酸刺激胰高血糖素释放，但不影响基础酶分泌。然而，在精氨酸存在的情况下，胆囊收缩素刺激的淀粉酶分泌显著受到抑制。精氨酸对胰腺外分泌的这种抑制作用可被胰高血糖素抗体阻断，但不能被非特异性γ球蛋白阻断。因此，外源性胰高血糖素在体外未能影响胰腺外分泌，但内源性释放的胰高血糖素或胰高血糖素样肽可抑制离体灌注胰腺中的淀粉酶释放。我们得出结论，胰高血糖素或胰高血糖素样肽可能是胰岛-腺泡轴中的一种介质。
