Kordula T, Bugno M, Lason W, Przewlocki R, Koj A
Jagiellonian University, Institute of Molecular Biology, Krakow, Poland.
Biochem Biophys Res Commun. 1994 May 30;201(1):222-7. doi: 10.1006/bbrc.1994.1692.
Three highly homologous serine protease inhibitors, SPI-1, SPI-2 and SPI-3 (contrapsins), are synthesized in rat liver. Their expression is regulated differently in healthy and inflamed animals. We found that interleukin 6 (IL-6), a major acute phase cytokine, and to a lesser extent leukemia inhibitory factor (LIF), both together with glucocorticoids, are responsible for the regulation of expression of the contrapsins in rat hepatocytes in primary culture. The effect of IL-6 is time- and dose-dependent. IL-1, TGF beta 1, HGF, PMA and IL-8 did not have any effect on contrapsin mRNA levels. We postulate that SPI-1, SPI-2 and SPI-3 belong to the class II acute phase proteins. Additionally, we show induction of SPI-3 mRNA in rat liver by in situ hybridization using a specific oligonucleotide probe.
三种高度同源的丝氨酸蛋白酶抑制剂，SPI-1、SPI-2和SPI-3（抗蛋白酶）在大鼠肝脏中合成。它们在健康动物和炎症动物中的表达调控方式不同。我们发现，主要的急性期细胞因子白细胞介素6（IL-6），以及程度较轻的白血病抑制因子（LIF），与糖皮质激素共同作用，负责原代培养的大鼠肝细胞中抗蛋白酶表达的调控。IL-6的作用具有时间和剂量依赖性。IL-1、转化生长因子β1（TGF beta 1）、肝细胞生长因子（HGF）、佛波酯（PMA）和IL-8对抗蛋白酶mRNA水平没有任何影响。我们推测SPI-1、SPI-2和SPI-3属于II类急性期蛋白。此外，我们使用特异性寡核苷酸探针通过原位杂交显示了大鼠肝脏中SPI-3 mRNA的诱导。
