使用图像分析测量核仁组成区嗜银蛋白（AgNORs）。
Measuring of AgNORs using image analysis.
作者信息
Hufnagl P, Guski H, Schulz H J
机构信息
Institut für Pathologie der Medizinischen Fakultät (Charité), Humboldt-Universität zu Berlin, Deutschland.
出版信息
Zentralbl Pathol. 1994 Mar;140(1):31-5.
A method is presented for the measurement of AgNORs in histological section using an image analysis system. Measurements are performed at the highest magnification (objective 100x) using a CCD camera. In the first step the AgNORs are segmented, counted and measured. In the second step the AgNOR clusters are segmented. Several features for the description of the AgNORs, the AgNOR clusters and their spatial distribution throughout the nucleus are given. As an internal reference, lymphocytes are used and the size features are given relative to the mean size of AgNORs in the lymphocytes. The reproducibility of the features was tested on a series of astrocytomas. This should be carried out with at least 150 tumor cells and 20 reference cells in order for accurate measurements.
本文介绍了一种使用图像分析系统测量组织学切片中银染核仁组织区（AgNORs）的方法。使用电荷耦合器件（CCD）相机在最高放大倍数（物镜100倍）下进行测量。第一步对AgNORs进行分割、计数和测量。第二步对AgNOR簇进行分割。给出了用于描述AgNORs、AgNOR簇及其在整个细胞核中的空间分布的几个特征。作为内部参照，使用淋巴细胞，并给出相对于淋巴细胞中AgNORs平均大小的尺寸特征。在一系列星形细胞瘤上测试了这些特征的可重复性。为了进行准确测量，应至少对150个肿瘤细胞和20个参照细胞进行此操作。
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