Salvadori D M, Ribeiro L R, Natarajan A T
MGC Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, State University of Leiden, The Netherlands.
Mutagenesis. 1994 Jan;9(1):53-7. doi: 10.1093/mutage/9.1.53.
The effect of beta-carotene on the frequencies of micronuclei (MN) induced in cytochalasin blocked binucleated Chinese hamster ovary cells (CHO) by a bifunctional alkylating agent mitomycin C (MMC), a monofunctional alkylating agent methyl methanesulphonate (MMS) and a radio-mimetic agent bleomycin (BLEO) was investigated. Four different modes of application of the combination of clastogens and beta-carotene were examined (pre-treatment, simultaneous treatment, pre- +simultaneous treatment and post-treatment). The results obtained showed no effect of beta-carotene on the frequencies of MN induced by MMS, a slight but not statistically significant reduction of MMC-induced MN only when beta-carotene was used in low concentrations (0.25 and 0.5 microM) and a potentiation of the clastogenicity of bleomycin by beta-carotene in three of the treatment regimes utilized, post-treatment being ineffective. On the basis of these results it can be concluded that the effect of beta-carotene on clastogenesis induced by chemicals depends on the type and mechanism of action of the clastogen used as well as the treatment protocol employed.
研究了β-胡萝卜素对用双功能烷化剂丝裂霉素C（MMC）、单功能烷化剂甲基磺酸甲酯（MMS）和拟放射性药物博来霉素（BLEO）诱导细胞松弛素阻断的双核中国仓鼠卵巢细胞（CHO）中微核（MN）频率的影响。研究了四种不同的致断裂剂与β-胡萝卜素联合应用的方式（预处理、同时处理、预处理+同时处理和后处理）。所得结果表明，β-胡萝卜素对MMS诱导的MN频率无影响；仅当β-胡萝卜素以低浓度（0.25和0.5 microM）使用时，MMC诱导的MN频率略有降低，但无统计学意义；在所用的三种处理方案中，β-胡萝卜素增强了博来霉素的断裂作用，后处理无效。基于这些结果可以得出结论，β-胡萝卜素对化学物质诱导的断裂作用的影响取决于所用致断裂剂的类型和作用机制以及所采用的处理方案。
