Peek R, Westphal J R, Pruijn G J, Van der Kemp A J, Van Venrooij W J
Department of Biochemistry, University of Nijmegen, The Netherlands.
Clin Exp Immunol. 1994 Jun;96(3):395-402. doi: 10.1111/j.1365-2249.1994.tb06041.x.
Antibodies to the RNA polymerase III transcription termination factor La are frequently found in the serum of patients with various autoimmune diseases. The mechanisms by which autoimmune responses are evoked remain largely obscure, but the presentation of autoantigens on the cell surface during stress conditions has been reported as a possible factor. In this study we analysed the effects of adenovirus infection on the binding of anti-La antibodies to the surface of several human cell lines and on the levels of the membrane-expressed glycoproteins HLA class I, CD44 and the CD3 complex. In addition, we studied the relative amount and the intracellular distribution of the La protein as well as its association with the major species of non-coding virus-associated (VAI) RNA. While immunofluorescence patterns revealed a redistribution and possibly cell surface expression of the La protein during infection, this could not be confirmed by other techniques. In contrast, surface levels of HLA class I proteins and CD3 complex were severely affected. The data suggest that the subcellular distribution of the La protein is not detectably influenced by adenovirus infection.
在各种自身免疫性疾病患者的血清中经常发现针对RNA聚合酶III转录终止因子La的抗体。引发自身免疫反应的机制在很大程度上仍不清楚，但据报道，应激条件下自身抗原在细胞表面的呈递是一个可能的因素。在本研究中，我们分析了腺病毒感染对几种人类细胞系表面抗La抗体结合以及膜表达糖蛋白HLA I类、CD44和CD3复合物水平的影响。此外，我们研究了La蛋白的相对含量和细胞内分布，以及它与主要种类的非编码病毒相关（VAI）RNA的关联。虽然免疫荧光模式显示感染期间La蛋白重新分布并可能在细胞表面表达，但其他技术无法证实这一点。相反，HLA I类蛋白和CD3复合物的表面水平受到严重影响。数据表明，腺病毒感染对La蛋白的亚细胞分布没有可检测到的影响。
