Green L K, Ansari M Q, Schwartz M R, Ro J Y, Alpert L C
Department of Pathology, Veterans Affairs Medical Center, Houston, Texas 77030.
Thorax. 1994 Jun;49(6):590-3. doi: 10.1136/thx.49.6.590.
Solitary lung nodules in humans caused by the dog parasite Dirofilaria immitis are steadily increasing in number. The organisms can be easily missed in haematoxylin and eosin stained tissue when they are degenerated and pale staining.
The value of Tinopal CBS-X (TCBS-X) and Calcofluor white (CFW), two rapid, inexpensive, simple non-specific fluorescent whitening stains, were assessed in the identification of these worms. Deparaffinised rehydrated tissue slides prepared from the pulmonary nodules were stained for one minute in 1% w/v aqueous solutions of TCBS-X or CFW, counterstained, coverslipped, and viewed with a fluorescent microscope.
The stains demonstrated the intact worm and worm fragments in 20 cases of pulmonary dirofilariasis collected from hospitals in Houston. The filariae and fragments of filariae stained bright green while the background tissue stained red, delineating the internal structures of the worm.
Dirofilariasis should be included in the differential diagnosis of subpleural masses, and non-specific fluorescent whitening stains can help in the rapid recognition of the fragmented organism in cytological or surgical material.
由犬恶丝虫引起的人类孤立性肺结节数量正在稳步增加。当虫体退化且染色浅时，在苏木精-伊红染色组织中很容易漏检这些虫体。
评估了两种快速、廉价、简单的非特异性荧光增白剂Tinopal CBS-X（TCBS-X）和荧光增白剂（CFW）在识别这些虫体方面的价值。将从肺结节制备的脱蜡复水组织切片在1%（重量/体积）的TCBS-X或CFW水溶液中染色1分钟，复染，封片，并用荧光显微镜观察。
这些染色剂在从休斯顿医院收集的20例肺丝虫病病例中显示出完整的虫体和虫体片段。丝虫及其片段染成亮绿色，而背景组织染成红色，勾勒出虫体的内部结构。
丝虫病应纳入胸膜下肿块的鉴别诊断，非特异性荧光增白剂有助于在细胞学或手术材料中快速识别破碎的虫体。
