Shen N, Ffrench P, Guyotat D, Ffrench M, Fiere D, Bryon P A, Dechavanne M
Haemostasis Laboratory, Pasteur Institute of Lyon, France.
Eur J Haematol. 1994 May;52(5):296-301. doi: 10.1111/j.1600-0609.1994.tb00099.x.
Endothelial cell activation during allogeneic bone marrow transplantation, mainly in acute graft-versus-host disease (aGvHD) was studied in 23 recipients and 5 controls using anti-von Willebrand factor (vWF) antibody, antibodies to endothelial leukocyte adhesion molecule-1 (ELAM-1), vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and anti-HLA-DQ antibody, by immunohistological staining of skin. vWF extravasation, ELAM-1 and VCAM-1 expression were present in most recipients with a cutaneous rash which was confirmed as an aGvHD by histological examination (documented aGvHD) (p = 0.005 for vWF extravasation and ELAM-1 expression and p = 0.03 for VCAM-1 expression in comparison with the controls). In recipients with a rash, the cases displaying vWF extravasation and ELAM-1 expression were significantly more numerous in those with a documented aGvHD than in those without histological features of aGvHD (p = 0.01). vWF extravasation and ELAM-1 occurred concomitantly (p < 0.01). This study demonstrates that, during the course of skin aGvHD following bone marrow transplantation, there is transient expression of ELAM-1 and VCAM-1 by endothelial cells and simultaneous vWF extravasation, indicating an intense inflammation with endothelial cell participation.
采用抗血管性血友病因子（vWF）抗体、抗内皮细胞白细胞黏附分子-1（ELAM-1）抗体、抗血管细胞黏附分子-1（VCAM-1）抗体、抗细胞间黏附分子-1（ICAM-1）抗体以及抗HLA-DQ抗体，通过皮肤免疫组织化学染色，对23例异基因骨髓移植受者和5例对照者在同种异体骨髓移植期间，主要是在急性移植物抗宿主病（aGvHD）中的内皮细胞活化情况进行了研究。vWF外渗、ELAM-1和VCAM-1表达见于大多数有皮疹的受者，经组织学检查证实为aGvHD（记录的aGvHD）（与对照组相比，vWF外渗和ELAM-1表达的p值为0.005，VCAM-1表达的p值为0.03）。在有皮疹的受者中，记录的aGvHD患者中出现vWF外渗和ELAM-1表达的病例明显多于无aGvHD组织学特征的患者（p = 0.01）。vWF外渗和ELAM-1同时出现（p < 0.01）。本研究表明，在骨髓移植后的皮肤aGvHD病程中，内皮细胞有ELAM-1和VCAM-1的短暂表达以及同时发生的vWF外渗，表明存在内皮细胞参与的强烈炎症反应。
