Neumann R, Leonhartsberger H, Knobler R, Hönigsmann H
Department of Dermatology, University of Vienna Medical School, Austria.
Arch Dermatol. 1994 Jul;130(7):879-83.
Immunohistochemical analysis using four monoclonal antibodies specific for endothelium was performed to evaluate the possible role the endothelium may play in the pathogenesis of port-wine stains. In 11 patients with port-wine stains, biopsy specimens were obtained from involved and normal skin. On frozen tissue sections, we studied and compared the distribution and staining pattern of PAL-E, anti-intercellular adhesion molecule-1 (ICAM-1), anti-endothelial leukocyte adhesion molecule-1 (ELAM-1), and anti-factor VIIIrAg (FVIIIrAg), all recognizing specific epitopes of vascular endothelial cells.
The PAL-E, anti-FVIIIrAg, and anti-ICAM-1 antibodies showed a similar distribution and staining pattern. The intensity of staining was equally strong with PAL-E and FVIIIrAg, while the expression of ICAM-1 was moderate. The ELAM-1 antibody exhibited only a weak expression in about 70% of evaluated specimens. No substantial differences in the intensity and distribution pattern of expression of these proteins could be demonstrated between normal skin and port-wine stains.
Our findings suggest that the abnormal vessel pathologic findings in port-wine stains are not due to defects associated with the endothelium. According to PAL-E antibody staining properties, port-wine stain vessels could be classified as capillaries and/or postcapillary venules and small veins.
使用四种对内皮细胞具有特异性的单克隆抗体进行免疫组织化学分析，以评估内皮细胞在葡萄酒色斑发病机制中可能发挥的作用。在11例葡萄酒色斑患者中，从病变皮肤和正常皮肤获取活检标本。在冷冻组织切片上，我们研究并比较了PAL-E、抗细胞间黏附分子-1（ICAM-1）、抗内皮白细胞黏附分子-1（ELAM-1）和抗因子VIII相关抗原（FVIIIrAg）的分布和染色模式，所有这些抗体均识别血管内皮细胞的特定表位。
PAL-E、抗FVIIIrAg和抗ICAM-1抗体显示出相似的分布和染色模式。PAL-E和FVIIIrAg的染色强度相同，而ICAM-1的表达为中度。ELAM-1抗体在约70%的评估标本中仅表现出弱表达。在正常皮肤和葡萄酒色斑之间，这些蛋白质表达的强度和分布模式没有显著差异。
我们的研究结果表明，葡萄酒色斑中异常血管的病理表现并非由于与内皮细胞相关的缺陷所致。根据PAL-E抗体的染色特性，葡萄酒色斑血管可分类为毛细血管和/或毛细血管后微静脉及小静脉。
