Brenner T, Soffer D, Shalit M, Levi-Schaffer F
Department of Neurology, Hadassah University Hospital, Jerusalem, Israel.
J Neurol Sci. 1994 Apr;122(2):210-3. doi: 10.1016/0022-510x(94)90300-x.
Mast cells (MC) have been implicated in the pathogenesis of experimental allergic encephalomyelitis (EAE). In order to further evaluate their role, several morphological and functional studies were performed. Semiquantitative counts of histological sections showed a significant reduction in MC numbers in EAE brains. In addition, a higher proportion of EAE MC (about 50-70%) appeared degranulated compared with about 20% degranulation in controls. Central nervous system (CNS) MC exhibited staining properties of connective tissue MC and about 98% of them, both in diseased and control rats, were located in the thalamus. They were not present in the spinal cord and did not relate to EAE lesions. In vitro incubation of peritoneal MC (of connective tissue phenotype) with either MBP, or with neuropeptides such as substance P or bradykinin resulted in release of beta-hexosaminidase and histamine. The latter responses were similar in both EAE and control rats. It is suggested that the decrease in number and in granular content of CNS MC in EAE may reflect prior in vivo activation. The fact that MC were activated by MBP and by neuropeptides in vitro suggests a possible mechanism of MC activation in EAE.
肥大细胞（MC）已被认为与实验性变态反应性脑脊髓炎（EAE）的发病机制有关。为了进一步评估它们的作用，进行了一些形态学和功能研究。组织学切片的半定量计数显示，EAE脑内的MC数量显著减少。此外，与对照组约20%的脱颗粒率相比，EAE的MC中有更高比例（约50 - 70%）出现脱颗粒。中枢神经系统（CNS）的MC表现出结缔组织MC的染色特性，在患病和对照大鼠中，约98%的MC位于丘脑。它们不存在于脊髓中，且与EAE病变无关。用髓鞘碱性蛋白（MBP）或神经肽如P物质或缓激肽对腹膜MC（结缔组织表型）进行体外孵育，会导致β - 己糖胺酶和组胺的释放。EAE大鼠和对照大鼠的后一种反应相似。有人提出，EAE中CNS的MC数量和颗粒内容物的减少可能反映了其在体内的预先激活。MC在体外被MBP和神经肽激活这一事实提示了EAE中MC激活的一种可能机制。
