Hill A W, Shears A L, Hibbitt K G
Immunology. 1978 Jan;34(1):131-6.
Experiments using the selective divalent cation chelator, ethylene glycol tetraacetic acid (EGTA) showed that the alternate complement pathway was involved in the bactericidal action of bovine serum on two strains of E. coli. The bactericidal system was shown, in experiments involving EGTA and epsilon-amino caproic acid, to be independent of Ca++ and the C1 unit of complement. The fixation of the complement components C3-C9 by endotoxin does not require a functional classical pathway ((C1, C4 and C2). In the case of one coliform strain (W1) however, there was an almost total dependence on a factor absorbable at 0 degrees with homologous bacteria, and which appeared to be a natural antibody. These results suggest that in certain circumstances in the cow, gamma globulins may be directly involved in the activation of the alternate complement pathway.
使用选择性二价阳离子螯合剂乙二醇四乙酸（EGTA）进行的实验表明，替代补体途径参与了牛血清对两株大肠杆菌的杀菌作用。在涉及EGTA和ε-氨基己酸的实验中，杀菌系统被证明独立于Ca++和补体的C1单位。内毒素对补体成分C3 - C9的固定不需要功能性经典途径（C1、C4和C2）。然而，对于一株大肠菌群菌株（W1），几乎完全依赖于一种可在0℃时被同源细菌吸收的因子，该因子似乎是一种天然抗体。这些结果表明，在奶牛的某些情况下，γ球蛋白可能直接参与替代补体途径的激活。
