Fujii J T, Knapp P E
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201.
Brain Res Dev Brain Res. 1994 Apr 15;78(2):217-25. doi: 10.1016/0165-3806(94)90029-9.
Cultures of dissociated Edinger Westphal nuclei, dissected from embryonic chick brainstems, were screened immunohistochemically for a variety of non-neuronal cell markers. In young cultures, small clusters of cells were stained by the oligodendrocyte-specific antibodies 04 and 01. In older cultures, larger groups of cells were 04 and 01 positive, sheets of myelin-like membrane were elaborated, and immunoreactivity for proteolipid protein appeared. This sequence resembles that observed in well-characterized rodent brain cultures and suggests that oligodendrocytes in chick Edinger Westphal cultures differentiate in a pattern similar to rodent oligodendrocytes in culture. Variable numbers of cells were immunoreactive for glial fibrillary acidic protein. Many vimentin positive cells were observed, some of which morphologically resembled flat astrocytes. Together with the widespread presence of vimentin, large patches of fibronectin-like immunoreactivity suggested the presence of fibroblasts and/or endothelial cells. An anti-thymocyte polyclonal antibody stained a subset of cobblestone-shaped cells, possibly endothelial cells, in both Edinger Westphal cultures and control cultures of skin fibroblasts. Staining for smooth muscle myosin was detected in several patches of cells, tentatively identifying them as pericytes or smooth muscle cells. In conclusion, Edinger Westphal cultures contain a diverse and varying population of non-neuronal cells loosely organized in large, overlapping islands of cell types and including oligodendrocytes, astrocytes, possibly fibroblasts, endothelial cells, pericytes and/or smooth muscle cells.
从胚胎期鸡脑干中分离出的动眼神经核进行解离培养，运用免疫组织化学方法对多种非神经元细胞标志物进行筛选。在早期培养物中，少突胶质细胞特异性抗体04和01可使小细胞簇染色。在后期培养物中，大量细胞呈04和01阳性，出现了片状髓磷脂样膜，且对蛋白脂蛋白产生免疫反应。这一序列与在特征明确的啮齿动物脑培养物中观察到的序列相似，表明鸡动眼神经核培养物中的少突胶质细胞以与培养的啮齿动物少突胶质细胞相似的模式分化。不同数量的细胞对胶质纤维酸性蛋白呈免疫反应。观察到许多波形蛋白阳性细胞，其中一些在形态上类似于扁平星形胶质细胞。与波形蛋白的广泛存在一起，大片纤维连接蛋白样免疫反应提示存在成纤维细胞和/或内皮细胞。一种抗胸腺细胞多克隆抗体在动眼神经核培养物和皮肤成纤维细胞对照培养物中均使一部分鹅卵石样细胞染色，这些细胞可能是内皮细胞。在几处细胞斑块中检测到平滑肌肌动蛋白染色，初步将它们鉴定为周细胞或平滑肌细胞。总之，动眼神经核培养物包含多种不同的非神经元细胞群体，这些细胞松散地组织在大型、重叠的细胞类型岛中，包括少突胶质细胞、星形胶质细胞、可能的成纤维细胞、内皮细胞、周细胞和/或平滑肌细胞。
