Newcombe J, Meeson A, Cuzner M L
Department of Neurochemistry, Institute of Neurology, London, England.
Neuropathol Appl Neurobiol. 1988 Nov-Dec;14(6):453-65. doi: 10.1111/j.1365-2990.1988.tb01337.x.
Primary cultures were established from autopsy or biopsy samples of normal adult human brain and characterized by immunocytochemical techniques. Initially, macrophages were the predominant cell type adhering to the substratum, but as their number fell that of glial cells increased. Oligodendrocytes comprised 30% of the glial population in white matter cultures, and their perikarya and elongated processes were immunostained with antibodies directed against galactocerebroside and four myelin proteins. In white and grey matter cultures, process-bearing astrocytes and small numbers of polygonal astrocytes were stained with antibodies against glial fibrillary acidic protein and glutamine synthetase. Fibroblasts started to appear at 3 weeks and proliferated to form a monolayer beneath glial cells by 5 weeks. Glia began to die in the 6th week. These primary cell cultures of white or grey matter can be used to study the properties of glial cells from normal or pathological adult human brain.
原代培养物取自正常成人脑的尸检或活检样本，并通过免疫细胞化学技术进行鉴定。最初，巨噬细胞是附着在基质上的主要细胞类型，但随着它们数量的减少，神经胶质细胞的数量增加。少突胶质细胞在白质培养物中占神经胶质细胞群体的30%，它们的胞体和细长突起用针对半乳糖脑苷脂和四种髓鞘蛋白的抗体进行免疫染色。在白质和灰质培养物中，带有突起的星形胶质细胞和少量多边形星形胶质细胞用针对胶质纤维酸性蛋白和谷氨酰胺合成酶的抗体进行染色。成纤维细胞在3周时开始出现，并在5周时增殖形成神经胶质细胞下方的单层。神经胶质细胞在第6周开始死亡。这些白质或灰质的原代细胞培养物可用于研究正常或病理状态下成人脑胶质细胞的特性。
