Adisa A
Department of Botany and Microbiology, Lagos State University, Apapa, Nigeria.
Nahrung. 1994;38(3):318-26. doi: 10.1002/food.19940380312.
Thirteen fungi including toxigenic Aspergillus nidulans and A. clavatus were isolated from the grains. The isolated fungi grew well at 25-30 degrees C. A. clavatus and A. nidulans were grown in liquid maize yeast extract medium and wheat yeast extract medium. Both fungi produced amylases on the two media and on the basal medium at 30 degrees C. During incubation more total reducing sugars were detected in maize grains than in wheat while non-reducing sugars were detected than reducing sugars in both grains. A. clavatus showed highest amylase activities at 25-30 degrees C and at a pH 7-8 while 30 degrees C and pH 6.0 were the optimum conditions for highest amylase activities of A. nidulans. During incubation with both fungi a decrease in the protein and carbohydrate contents of both grains was recorded while more fat was accumulated in the grains.
从谷物中分离出了13种真菌，包括产毒的构巢曲霉和棒曲霉。分离出的真菌在25至30摄氏度下生长良好。棒曲霉和构巢曲霉在液态玉米酵母提取物培养基和小麦酵母提取物培养基中培养。两种真菌在这两种培养基以及30摄氏度的基础培养基上均产生淀粉酶。在培养过程中，玉米籽粒中检测到的总还原糖比小麦中的多，而两种籽粒中检测到的非还原糖比还原糖多。棒曲霉在25至30摄氏度以及pH值为7至8时显示出最高的淀粉酶活性，而30摄氏度和pH值6.0是构巢曲霉淀粉酶活性最高的最佳条件。在与两种真菌共同培养期间，记录到两种籽粒的蛋白质和碳水化合物含量均下降，而籽粒中积累了更多的脂肪。
